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KMID : 1161520180220010007
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Animal Cells and Systems
2018 Volume.22 No. 1 p.7 ~ p.14
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Phosphatidylcholine regulates NF-κB activation in attenuation of LPS-induced inflammation: evidence from in vitro study
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Chen Meijuan
Pan Hongying
Dai Yining
Zhang Jiajie
Tong Yongxi
Huang Yicheng
Wang Mingshan
Huang Haijun
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Abstract
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Phosphatidylcholine (PC) has been demonstrated as anti-inflammatory and antioxidant/pro-oxidant molecules. In this study, we investigated the protective effects of PC on inflammatory bowel disease (IBD) caused by lipopolysaccharide (LPS)-induced injury in intestinal epithelia cells. The IEC-6 cells (intestinal epithelia cells) were stimulated with LPS (1 μg/mL) for 24 h with or without PC pretreatment, in the next steps: (1) the level of the inflammatory cytokine tumor necrosis factor (TNF)-α was measured with ELISA; (2) the nuclear translocation and phosphorylation of NF-κB was investigated with Western blot, EMSA, immunofluorence assay; (3) the protein phosporylation levels in MAPK signaling pathway were detected with Western blot method. The results showed: (1) compared with the normal group, 10 and 20?μg/mL of PC significantly inhibited the production and activation of TNF-α, (P??
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KEYWORD
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Phosphatidylcholine, LPS-induced injury, tTNF-α secretion and activation, p65 nuclear translocation, MAPKs signal pathway
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